FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085783
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG400.00621999453.99952
TCTGTCG8950.039.418638
GTCTCGC9450.032.0211941
GTCTTGC18000.032.0211941
TCGCTCT10600.031.924234
CTGTCGC12200.031.2783999
GTGTAAC7100.029.4279291
GGTATAC4200.029.1621591
GTATTAG5800.028.5706371
GTACGGG1856.1102364E-627.2612881
GTATTAT4550.026.9189171
GGTTACG1358.3866785E-426.684331
CGCTCTG11200.025.7140455
GTCTTAC8000.025.2166921
TTACACT10850.024.5527654
GTGTAAG10150.024.1342011
TCTCGCT12100.023.801432
GTATTAC5150.023.7827321
GTGTTAG15600.023.554051
TAATACT7100.023.3237254
Produced by FastQC (version 0.11.5)