FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623052
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7050.046.975958
GTCTTGC14650.039.432921
GTCTCGC7150.038.3781551
TCGCTCT8000.035.997884
CTGTCGC9750.035.444069
GTCGACC1251.3275394E-534.5579578
GTCTTAC7050.033.801141
GTGTTAC6450.033.5867581
GTATTAG5400.030.7567621
GTGTAAC4700.030.7283121
GGTCGAC1453.662073E-529.7913467
GTGTTGC9200.029.0415941
TCCGTAC1000.006285329328.7991893
GTTTCAC23900.028.6184373
TTCACCG4600.028.1722495
GGTATAC3351.8189894E-1228.0223851
TCTCGCT8850.027.6602362
GTATATA5600.027.0793251
GTATTAT4800.027.0793231
CTCGCTC9600.026.9992373
Produced by FastQC (version 0.11.5)