FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780147
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT7700.044.881884
TCTGTCG7350.042.1212928
GTCTCGC7550.040.1669581
GTCTTGC15550.033.8968281
GTATTAT4800.033.093911
CTCGCTC9950.032.5628363
CGCTCTG9800.032.325645
TCTCGCT10050.031.5224062
CTGTCGC10150.030.5016259
GTATCGC1453.5904806E-529.8778841
GTCTTAC6050.027.4497911
TTGCTCT20000.025.9192874
GTACCGC1400.001023843825.787461
GTGTTAG10700.025.6428571
GTGTTAC6800.025.4840771
GTTTCAC23000.025.3565353
GTATAAT5150.025.236661
GGGTTTC26250.024.4808941
GTGTACA11300.024.281291
GGTATAC3001.7846105E-824.0682961
Produced by FastQC (version 0.11.5)