FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780147
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC1053.9932074E-641.1712381
GTCTCGC8650.037.4824831
GTCTTGC15550.033.823961
TCGCTCT9250.032.691474
TCTGTCG7450.028.9929148
GCGCGAT4800.028.4996329
GTTTCAC23650.027.7036613
TCTCGCT10400.027.6919522
CGCTCTG11400.027.1575455
GTATTAA4300.026.809181
GGTATAC3255.4569682E-1126.6029551
GTATTAT5150.026.5814321
TATACTG4900.026.4486395
GTATTAC3006.311893E-1026.4182111
CTCGCTC10750.024.7810763
GTCCTAC4950.024.744331
GTCTCAC21050.024.6440661
GTGTAGC8900.024.2864061
GTACAGG10450.024.1314661
TTGCTCT20600.024.1161964
Produced by FastQC (version 0.11.5)