FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690859
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7500.041.292951
TCTGTCG7650.040.4701168
TCGCTCT8550.037.0510984
GTACGGC850.002832797833.8929821
CGCTCTG10200.033.880965
CTCGCTC9200.033.6507873
GATCGTT2155.2568794E-1033.4879849
TCTCGCT9850.032.1611062
GTGTTAC7600.031.2729641
GTCTTAC6150.029.2774731
GTGTACG2502.6284397E-928.8090361
GTATCGG1504.6032634E-528.8090341
CTGTCGC11200.028.2853819
GTCTCAC19100.027.5269551
GTCTTGC16150.027.2035791
GTTTCAC25700.026.6136863
TAGTACT3255.638867E-1126.5835194
TAACACT7050.026.5520974
GTGTAAT6250.026.504311
GTATAAC3306.730261E-1126.1900311
Produced by FastQC (version 0.11.5)