FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335214
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC5100.048.139261
TCTGTCG5150.047.5326468
TCGCTCT5950.045.98194
GTCTTGC11550.038.13631
GTATAGG3900.033.3271831
TCTCGCT7000.032.9145932
CTCGCTC7100.032.4510083
GTATAAC2051.1847078E-831.7014681
GTATTAC3250.031.1053681
CTGTCGC8200.030.7308439
GTGCGAA1204.1555622E-430.087041
CGCTCTG8200.028.0967715
GTTTCAC18400.027.391563
GTCTCAC16100.027.358651
GGTATAC2654.7602953E-927.248641
GTACTAT2955.0567905E-1026.925351
GTACAGA7000.026.8204461
GTCTTAC5150.026.6401751
GGCGTAA1400.001023377825.788891
GTGCGAC1400.001023377825.788891
Produced by FastQC (version 0.11.5)