FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474605
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7400.040.8761141
TCTGTCG5850.039.383448
GTCTTGC13350.036.144781
TCGCTCT8800.035.1807674
GTACCGC850.00283321433.891681
GTACTAT2155.2386895E-1033.4975931
CGCTCTG8400.033.427575
TACCCGT1352.2471739E-531.9990445
GTATTAC3500.030.865641
TCTCGCT9450.030.4752772
GTCTCGA14150.030.0203046
GTATTAG4400.029.4626581
TACACTG10000.028.079165
CTCGATC6700.027.9394638
CTCGCTC10100.027.8011473
TCGATCT6650.027.066869
GTGCGGT5100.026.8309151
GTATATA6000.026.407271
CTGTCGC9000.026.399219
TTGCTCT19100.026.3866464
Produced by FastQC (version 0.11.5)