FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745935
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.042.7960558
GTCTTGC24900.039.040851
GTCTCGC11450.036.4760131
TCGCTCT13000.034.3457264
TCTCGCT13600.031.7685342
CTGTCGC15100.030.9959839
CGCTCTG15600.027.6956445
CTCGCTC15400.027.587743
TTGCTCT35250.026.3545114
GTGTAGC11350.025.3775081
GTATTAG9650.025.3709341
GTCTTAC9950.025.3296851
GTCTCAC30750.025.2908541
GGGTTTC41450.025.0162831
CTTGCTC32950.024.6949353
TCTGTCA34950.024.5170848
GTTTCAC39150.024.094863
GTGTATA13800.024.0028921
GTGTTAG19600.023.880431
GTATAGT7250.023.8373571
Produced by FastQC (version 0.11.5)