FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745935
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.039.3296668
GTCTCGC12200.034.252571
TCGCTCT12950.033.915664
GTATAAC4300.031.8353941
GTCTTGC24000.030.620621
GTATTAC6050.029.7721161
GTGTAAC9150.028.3469581
CTCGCTC15700.027.9750213
TCTCGCT16050.027.813582
GTCGTAG2156.474911E-726.8087541
CGCTCTG16450.026.2618685
GTCTTAC10550.025.9511261
GAGTGCA72650.025.5696246
TTGCTCT33300.025.513974
GTGTTAG17000.024.5812591
GTATTAT8300.024.3055251
GGAGTGC66700.024.0723935
GTTTCAC35000.023.8632833
ATAGTAC5450.023.7802413
GTACTAT5300.023.1099011
Produced by FastQC (version 0.11.5)