FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625561
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9400.049.7899328
GTCTCGC10750.038.8493581
TCGCTCT13100.034.6304664
GTCTTGC24950.032.323011
CGCTCTG13300.031.9421925
CTGTCGC13550.031.8836469
GTATTAC5700.031.581261
TCTCGCT13050.031.450582
TACGACT1204.2245665E-430.0039144
GTTACCC10850.028.5366543
CTCGCTC14650.028.507213
GTGTTAG17400.026.48471
GGTATAC5450.026.4239541
GTGTTAC10000.025.2018451
TTGCTCT35050.024.6537154
GTATAAA10850.024.5547941
GTATTAG10000.024.4817921
GTTTCAC40900.024.295183
TACCCCG3001.8388164E-824.001765
GTATAAC5400.024.0017591
Produced by FastQC (version 0.11.5)