FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614369
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10800.042.0023888
TCGCTCT11150.039.391624
GTCTTGC23250.036.2308541
CTGTCGC14050.034.336479
GTCTCGC11700.033.844841
CTCGCTC13550.032.4120253
TCTCGCT13750.031.4169642
GTATAAT7200.029.9988351
CGCTCTG14750.027.3366245
GTCTTAC9400.026.0415421
GTATTAC6450.025.6734241
GTTTCAC39800.025.5065483
GGGTTTC43100.025.224081
GTGTAAC8450.024.7091031
TTGCTCT33850.024.6744924
GTGTAAA14400.024.4990481
TACTCCG2401.6984704E-624.0009045
GTATTAT7200.023.9990671
GTATTAA7250.023.8335571
TAATACT6950.023.8282384
Produced by FastQC (version 0.11.5)