FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775191
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11200.039.8596428
GTCTTGC25750.034.6721421
GTCTCGC14000.030.857411
TCGCTCT14400.028.50234
CTGTCGC16300.028.2717119
GTGTAAT11350.027.277771
GTATTAA7450.027.0606371
GTCTCAC32250.027.0141871
TCTCGCT15950.026.633462
GTGTTAG19600.026.4492071
TTCACCG8100.025.7793945
GTACTAT5950.025.4119851
GTGTTAA9400.025.2768151
TTGCTCT33550.025.1108194
GTTTCAC42650.024.647343
GTGTAAA13800.024.521951
GGGTAAC9150.024.3936521
GTATTAT6850.024.1753921
CGCTCTG16800.024.0015055
GTGTATA14000.023.6573491
Produced by FastQC (version 0.11.5)