FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859437
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT32450.17451518927503326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8450.029.824841
GTACCGT1753.983534E-628.8022771
GTATTAA3555.456968E-1226.368281
GTATTAG4353.6379788E-1223.1742441
TCTGTCG6600.021.8181448
GTACTAC2654.0140076E-621.7375661
TCGCTCT9000.021.602294
GTCTAAT3355.918264E-821.4942361
GTACTAA2353.912463E-521.4485021
TCTCGCT10450.020.671492
GTCCTAC3951.5295882E-820.0522171
GTCCTAA4702.6011548E-1019.9164661
ACACTTA4753.0013325E-1019.706296
GGTAAAT6650.019.4902611
GTCTTGC16000.019.351531
GTCTAGT6350.019.2771151
CCTATAC3001.1736307E-519.2015173
GTACAAG5709.094947E-1218.9488661
TGGTCTC32650.018.5237059
CTCGCTC10900.018.4968743
Produced by FastQC (version 0.11.5)