FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927560
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12100.041.6552058
GTCTTGC27150.035.8049241
GTCTCGC13700.034.1642341
TCGCTCT13350.032.9040764
GGTATAC5600.032.146291
GTATTAG9400.029.8755281
GTATTAT7000.029.8317581
CTGTCGC17350.028.6355979
TTGCTCT35250.028.6002624
TCTCGCT15000.028.3230232
GTATATA12100.027.9699271
GTCTTAC9600.027.7529641
GTTTCAC44450.027.5394973
GTGTAGA16000.026.1027891
GTATTAC6400.025.8777621
CTCGCTC15600.025.8489113
ACCGTGT10400.025.6168068
GTGTTAT10450.025.4955461
CGCTCTG15550.025.4689585
GTGTTAC12550.025.2457221
Produced by FastQC (version 0.11.5)