FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2871000
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12300.046.2454648
GTCTCGC12400.038.903861
CTGTCGC16200.035.1122979
GTCTTGC25150.033.495581
TCGCTCT14500.032.7746624
TTACGCA2151.9379513E-830.1416024
TCTCGCT15550.030.0969562
GTCTTAC11950.028.3184531
GTATTAT7400.028.2166771
CGCTCTG16500.027.055465
GTATAAT8000.027.0004431
CTCGCTC16850.026.920323
GTGTAAT12050.025.0958461
GTCTCAC34900.024.9631581
GTCCTAC9700.024.4952451
GTATTAA8850.024.4071791
GTGTATA15650.024.3837851
TTGCTCT36600.024.198384
TTATACT8750.023.8644924
GTTTCAC48400.023.8020423
Produced by FastQC (version 0.11.5)