FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2871000
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12950.048.923448
GTCTCGC13400.044.63531
TCGCTCT15500.039.019524
CTGTCGC18750.035.325699
GTATTAA8200.034.2732961
TCTCGCT18000.033.600142
CTCGCTC18700.031.5723273
CGCTCTG17350.030.7090635
GTCTTGC26500.030.1843781
GTATTAG9900.029.1158811
GTCTTAC11400.028.445451
GTACTAC5300.027.1931341
GTGTTAC12050.025.7150021
GTACAAG11200.024.4495411
TTACACT15150.024.2377284
GTGTAAC9300.024.02061
GTCTCAC36950.023.5980471
GTATAGG7750.023.2457451
TAATACT8700.023.1725124
TTGCTCT37700.023.108854
Produced by FastQC (version 0.11.5)