FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624809
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12250.039.381928
GTCTCGC11650.038.936441
TCGCTCT13000.038.7730034
GTCTTGC26050.034.549671
CTGTCGC15050.034.447229
CTCGCTC14750.030.753193
TCTCGCT14950.029.3785442
CGCTCTG15000.027.8416445
GGTATAC5450.027.7437021
TTATCCG1607.158959E-527.0015985
GTCTTAC10450.025.4933591
TTGCTCT32950.024.9128934
GGGTTTC43950.024.7377221
GTATAGA8000.024.3005121
GTTTCAC42250.023.6880723
GTATTAA7450.023.195121
GTATTAT8100.023.1115951
CTTGCTC33400.022.8507793
GTATAAT7600.022.737321
GTGTTAC12100.022.6120471
Produced by FastQC (version 0.11.5)