FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624809
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11300.037.592458
GTCTCGC13350.037.2477871
TCGCTCT12900.036.2799954
GTATTAG9400.033.7331921
GTCTTGC25250.031.395251
GTATTAT8200.029.8811781
TTCACCG6450.027.9076885
CTGTCGC17150.027.2882869
GTGTTAG17600.026.615421
CGCTCTG14900.026.0946245
TCTCGCT16900.025.9883372
GTACTAA5050.025.6870231
GGGTTTC41750.025.2016531
CTCGCTC16100.025.0441193
GTTTCAC38450.024.3439243
GTATAGA8000.024.32241
GTGTTAC11050.024.130821
GTATTGG7800.024.0221211
GTATAAG7950.023.5688761
GTCTCAC29400.023.286751
Produced by FastQC (version 0.11.5)