FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950048
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8000.042.2991038
GTCTTGC16900.035.4684221
GGTATAC4400.034.468081
CTGTCGC11050.033.2300649
CGTTTCG1000.00628542128.799399
GTCTCGC8300.028.7135121
GTACTAC4100.028.182961
GCGTAGA1555.6506877E-527.9556791
TCGCTCT9900.027.6357774
GTATTAT5500.027.5744651
TTGCTCT25450.027.158564
TAATACG1358.420017E-426.6661034
GTGTAAC6850.025.302951
TTCACCG5500.024.8722025
GTATAAA7600.024.7064441
TTACACT9400.024.5101184
GTGTTAG14250.024.3263451
GGCTAGA13100.024.2567081
ATAGTAC5100.023.9994933
GTCTCAC23800.023.9717981
Produced by FastQC (version 0.11.5)