FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965893
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8850.053.6932148
CTGTCGC11300.044.600369
GTCTCGC7700.040.3162421
GTCTTGC17700.036.3010371
TCGCTCT8950.034.5928424
TCTCGCT9650.032.083522
CGCTCTG9700.031.9173285
GTATAAC3700.031.2191141
GCGTTAC1204.161231E-430.0809151
CTCGCTC9800.028.6536143
GTGTTAC9050.027.920411
GTGTAAA12450.027.2540341
TTGCTCT25900.026.1317754
GTACTAT4600.025.1110231
ACCGTGT6200.024.3863268
AACCGTA1801.5941799E-423.9998517
TATACTG5450.023.7796695
GTATTAG7300.023.7350791
GTGTTAG13700.023.713421
CTTGCTC24700.023.3202883
Produced by FastQC (version 0.11.5)