FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965893
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8350.038.8272171
GTCTTGC17400.036.4370841
TCGCTCT10150.034.0467764
ACCGTGT6650.033.5614628
TCTGTCG8800.033.5430038
GCGTAAT1102.5285277E-432.7482071
GTATAAC3150.032.020471
TAATACT6200.031.3525524
TCTCGCT10500.029.4835642
TTATACT5950.027.82994
TTCACCG6250.027.6459835
CGCTCTG10200.027.5274055
CCGTGTT6300.026.2837949
CTCGCTC11250.026.2380853
GGGTTTC33200.025.6067311
GTCTCAC24800.025.564731
CTGTCGC14550.024.2456559
GTTTCAC30250.024.0379163
GTACTAG4201.8189894E-1224.0153521
TTGCTCT23950.023.1465384
Produced by FastQC (version 0.11.5)