FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342102
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9850.042.509961
TCTGTCG8450.041.7511568
TCGCTCT10400.035.9989554
TCTCGCT11400.035.9989552
GTCTTGC21250.035.672151
CTCGCTC11650.032.754413
CGCTCTG11900.031.461275
GGTATAC4950.030.6276051
CTGTCGC13050.029.7928739
GTTTCAC34200.029.4728263
GTCTTAC9200.029.0343971
GTCCGAC1255.2852946E-428.8774571
GTGTTAT9550.027.9703121
GGGTTTC40450.027.128391
GTACGGA2456.7162546E-826.5201131
GTGTAAT9150.026.037051
GTATAGT6150.025.8253691
TCTGTCA28500.025.7682238
GTCTCAC27700.025.2808061
TACCCCG2851.0670192E-825.2624235
Produced by FastQC (version 0.11.5)