FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342102
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11100.055.825111
TCGCTCT12550.043.6003654
CGCTCTG12700.041.3846635
TCTCGCT13400.039.222782
TCTGTCG11200.039.2140168
CTCGCTC14750.035.1447753
GTCTTGC20850.031.4477461
GTATAAG5950.030.2745151
GTGTTAG17200.029.7429541
GTATCGC1255.3458055E-428.821341
CTGTCGC16600.027.7588469
GTATTAA6750.026.6864261
GGGTTTC36200.026.4726411
GTATAAA8500.026.2782781
GTATATA8250.026.2012181
GTTTCAC33700.026.0645483
GTCTCAC26900.025.1784921
GTCTAGA10600.025.1506961
GTGTTAC10350.025.0620371
ACCGTGT7750.024.1546758
Produced by FastQC (version 0.11.5)