FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201772
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10050.045.850488
GTCTCGC10250.044.379381
TCGCTCT10500.041.827384
TCTCGCT11850.038.277392
CTCGCTC11950.036.1495933
CTGTCGC12450.035.855219
GTCTTGC22000.034.461261
CGCTCTG13200.033.8172155
GTATTAA5950.031.5515651
CGTTTCG1402.8813121E-530.8569629
TACCCGT1652.521123E-630.5445825
GTATTAC5150.029.442631
GTCTTAC7550.028.6905461
GTGTTAC10650.028.4750351
GTATTAT5850.028.3881111
GTATTGC6950.028.0506881
TACACTG13800.027.6513825
GTGTTAG14150.027.555091
TTGCTCT26700.027.5048334
GCGTAAT1358.280975E-426.7424261
Produced by FastQC (version 0.11.5)