FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201772
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG657.596287E-444.3037459
TCGCTCT9900.038.5411454
GTCTCGC9650.037.3371431
TCTGTCG10500.034.282668
GTCTTGC20450.032.066121
TCTCGCT11400.029.6808912
GTGTAAT10400.029.1014291
GTCTCAC24650.028.9412061
GTATTAC4900.027.9418961
CTCGCTC12450.027.1776853
CGCTCTG12050.025.6900755
GTTCTAC7050.025.5534321
GGAGTGC50850.025.4838775
GAGTGCA57500.024.7902726
TTATACT6400.024.7472324
GGGTTTC33700.024.5904391
GGTATAC4700.024.5312961
CGTATGG1500.001557322523.9973162
GTGTATA14850.023.77761
TTGCTCT30650.023.7232864
Produced by FastQC (version 0.11.5)