FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966175
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8950.047.4624758
GTCTCGC9000.044.9165041
TCGCTCT9200.043.0423744
CTGTCGC11600.035.999089
TCTCGCT10450.035.1378562
CGCTCTG9850.035.0853965
GTCTTGC18750.033.879881
GTATTAT6100.033.1351241
CTCGCTC12350.028.566073
CCGTTAT1050.00787460627.4998991
GTGTCGC2654.778485E-927.2404671
GTATTAC5050.027.1595571
GGGTTTC30550.026.93731
GTGTAAA10750.026.8603691
GTGTTAA8600.026.020981
GTCTCAC24350.025.7917461
TTGCTCT28150.025.0650794
GTATTAG6350.025.0097521
TCTGTCA24550.024.3415368
GTTTCAC30700.024.1557333
Produced by FastQC (version 0.11.5)