FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966175
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8750.037.849568
GTCTCGC7850.035.805361
GTCTTGC19200.034.1580661
GTATAAG5200.029.1050951
CGCTCTG9900.029.0894785
TCGCTCT9750.028.7985784
GTCCTAC5800.028.579391
GTATAGC4450.027.532271
TATACTG6850.027.327125
GTACTAT4500.027.2263551
GTACTAC4050.026.6925051
GTGTACG2851.05555955E-825.2876361
CTCGCTC10950.024.9850713
TTGCTCT25500.024.8458354
AACCCGT2901.2838427E-824.8263636
TCTCGCT10450.024.8026072
TATAATA4950.024.7260552
GTGTAAT8750.024.7096331
TTCACCG5850.024.6141725
CTTGCTC25800.024.5569293
Produced by FastQC (version 0.11.5)