FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102385
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7750.046.4506078
GTCTCGC9150.038.6641271
TCGCTCT9700.037.8548554
CTGTCGC10750.036.8364149
TCTCGCT11250.033.2792782
CTCGCTC11500.030.6775973
GTATAAT7550.030.6010461
GTCTTGC21150.029.699021
CGCTCTG10850.029.1975275
GTATTAT7950.029.0613691
GTATTAA6250.028.8797381
GACCCGT2207.960516E-726.1812526
GTATTAG7750.026.0849251
GTGTATA13700.025.8231221
GTGTAGA12950.025.6461011
GTATAGG5350.025.6408881
GTGTAAC7700.025.3166521
GTGTTAC9600.024.8185251
GTGTAAA14700.024.0664481
CGAGTAC2101.6420578E-523.9994813
Produced by FastQC (version 0.11.5)