FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102385
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10450.049.6478651
TCGCTCT10800.043.329264
TCTGTCG10500.038.3963938
TCTCGCT13150.037.228442
CTCGCTC12850.036.4168133
GTCTTGC20200.036.3859021
CGCTCTG13150.035.0385325
GTGTTAG14900.031.9184651
GTATTAG7500.030.7448981
TTACACT10700.028.9318584
GGTATAC5300.028.5514241
CTGTCGC15800.027.7948579
TACACTG14650.025.5539115
GTGTAAC7050.025.5526081
GTTAGCC11650.025.3366743
GGGTTTC34050.024.9717661
GTATTAC6400.024.7700611
GTTTCAC32300.024.517823
GTGTAAG11550.024.3313941
TGTTAGC13400.023.6395682
Produced by FastQC (version 0.11.5)