FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199717
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10550.040.2988971
TCTGTCG9850.038.7380878
TCGCTCT11200.037.28284
GTCTTGC20000.034.9490471
TTCACCG7000.032.9117165
CTCGCTC12800.030.9350813
CGCTCTG13700.029.4283585
TCTCGCT13750.028.2741552
CTGTCGC15000.027.3578629
GTATTAC4550.026.9234751
GATTCGT1358.4231625E-426.6645836
GGGTTTC37100.026.0270251
GTATATA10100.025.6847131
GTTTCAC33850.025.309693
GTCTCAC25500.024.8677271
ACCGTGT9000.024.7980618
GTACGGT1751.3094192E-424.706251
GTATAAC3801.4551915E-1124.652071
TAATACT7750.024.1529524
GTCCTAT6600.024.0199641
Produced by FastQC (version 0.11.5)