FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958363
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7850.047.6930858
GTCTCGC9100.044.4336551
TCGCTCT9150.043.2776154
TCTCGCT9700.039.3392332
GTCTTGC17550.039.085161
CTGTCGC10550.036.8521659
CTCGCTC10850.035.8332143
CGCTCTG10150.034.7577555
GTATTAG5850.032.090971
GTTTCAC27800.030.819383
GTCTTAC7350.030.4536781
TTGCTCT24800.029.9024834
TCTATCG1453.6616002E-529.7923648
GGGTTTC30000.029.603921
GCGTAAA1000.00619822228.8818761
GTACTAT3650.027.6949481
CTTGCTC23100.027.427893
CTGTTGC20200.027.0884349
GTGTTAG14250.026.8550761
ACCATGT24750.026.472078
Produced by FastQC (version 0.11.5)