FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958363
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8800.047.451818
GTCTCGC10200.043.0893441
TCGCTCT9750.042.82834
CTCGCTC11500.037.563053
GTCTTGC18250.037.1112171
TCTCGCT12500.033.982042
CGCTCTG12100.033.3203935
CTGTCGC13800.031.8242539
GTCTTAC7100.030.44411
GTGTTAG13300.029.2538051
TATACCG1000.006286546628.798345
TTGCTCT24250.028.204564
ACGGTTC1555.7710167E-527.8693628
CTTGCTC24800.027.5790563
TCTGTTG20250.027.3762028
GTTTCAC29350.027.2284133
GTATTCG1400.001036702825.7325151
AACGGTT1400.001041347925.7128037
GTGTAAA11500.025.687761
GTCTCAC22450.025.6752031
Produced by FastQC (version 0.11.5)