FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099200
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.050.2601978
GTCTCGC9750.043.5959131
GTCTTGC20000.036.742491
CTGTCGC16000.035.54729
TCGCTCT11850.031.59324
GTATTAG6350.030.6329211
TCTCGCT13200.029.9983522
CTCGCTC13650.028.4819533
CGCTCTG13700.027.8524865
TTGCTCT27350.027.1136864
ATAATAC5950.026.6203883
GTTTCGC4350.026.4813063
GGTTAAC3850.026.1978531
TAATACT5350.025.568694
GTGTTAC8900.025.0940111
GTCTCAC23600.025.0322721
CTTGCTC28400.024.8437083
GTCTTAC7500.024.0146981
GCGTAAT1500.001550746824.0146961
GCTCTGT48950.023.974176
Produced by FastQC (version 0.11.5)