FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618531
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.048.8337758
GTCTTGC24950.041.2822341
CTGTCGC16050.036.7821779
TCGCTCT11450.033.956284
GTCTCGC11950.033.753421
GTATAGG7050.029.628291
CTCGCTC13450.029.4423313
TTGCTCT33600.028.285674
GTATTAA8200.027.2298681
TCTCGCT15350.026.741222
TCTGTCA33000.026.3994528
GGGTAAC9100.025.3283141
GTATAGT5700.025.272771
CTTGCTC33100.025.2325883
GTGTTAT9750.025.1172451
GTATTAG7750.025.0934161
GTCTCAC31050.024.8210351
GTTTCAC38900.024.4318393
GTATTAC5050.024.2468471
TCTTGCT36800.024.0697772
Produced by FastQC (version 0.11.5)