FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992214
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG657.5803505E-444.323161
GTCTCGC14100.041.3761441
TCGCTCT16350.034.7906654
TCTGTCG12550.033.848548
TCTCGCT17700.031.7356432
GTATTAG10450.029.6371361
GTCTTGC27350.028.9680631
CTGTCGC15900.028.5282489
CGCTCTG18100.027.8466435
CTCGCTC19150.027.4477983
TAGGGCG2851.0664735E-825.2643744
GGGTTTC52000.024.9317781
GTGTTAC13200.024.5540241
GTGTTAT12700.024.3864631
GGCTAGA18700.023.8799921
GTATAGG8000.023.4081691
GTACGCC1851.9191847E-423.3524743
GGTATAC6200.023.2339151
GTTTCAC49150.022.8535923
GTGTTAG22000.022.58971
Produced by FastQC (version 0.11.5)