FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221105
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10600.044.178661
TCTGTCG8700.043.865898
CTGTCGC12200.033.049089
TCTCGCT13700.032.597792
TCGCTCT11650.032.1401374
GGCGTAC900.003742743732.0200921
GTCGTAC1153.2822453E-431.3241
CGCTCTG11800.031.1213555
GTCTTGC22850.030.8990361
GTCTTAC8450.030.6938151
GTGTAAT8700.028.9837021
ACCGTGT6750.028.802518
CTCGCTC13300.027.6114273
GTGTTAG13900.027.4704721
TCGTACA1856.121745E-627.2548222
GTATAGG4900.026.4655861
GTCTCAC26250.025.7990441
CTCGATC9800.025.7165268
CCGTCGG1701.0815498E-425.411699
TCGATCT9950.025.326569
Produced by FastQC (version 0.11.5)