FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104736
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC20650.036.047871
GTCTCGC10500.035.791221
CTGTCGC9300.034.0626379
TCTGTCG8000.033.2981648
TCGCTCT10800.032.6648674
GCGCGAT5850.029.5368339
TCTCGCT11550.028.0830942
CGCTCTG12000.026.9985125
CTCGCTC11600.026.714843
GTATTAG6500.026.6845781
GTACGAC1100.00982445126.2802681
GTATTAC4850.025.332011
GTACTAT5300.024.5447771
GTTTCAC35250.024.5337683
GGGTTTC40150.024.3002471
TTGCTCT30200.023.8397464
GTCCTAC6800.023.3817081
GGTATAC4403.6379788E-1222.9952341
GTACTAC4455.456968E-1222.736861
GTGTTAG16000.022.5846041
Produced by FastQC (version 0.11.5)