FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104736
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8900.044.495198
GTCTCGC8750.039.5185171
CTGTCGC10700.039.026899
GTCTTGC20000.033.4981161
TCGCTCT11550.031.170884
GTATAAT6500.027.707291
TCTCGCT11800.026.8556022
CTCGCTC11600.026.6914083
GTGTTAG14600.025.6577111
GTGTAAC6850.025.239931
CGCTCTG12150.024.8905245
GGGCGTA2051.3565748E-524.598671
GTATTAT5600.024.441791
GTCTTAC9150.023.6193311
GGTACGG1851.9156061E-423.3584372
GTATTAA6200.023.2383751
GTGTTAA9350.023.1141031
TAATACT6300.022.8586434
GTCTCGA18250.022.4891476
CTCGATC8350.022.4195678
Produced by FastQC (version 0.11.5)