FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431301
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12250.044.6669358
GTCTCGC16000.042.9196431
TCGCTCT15250.038.2405244
TCTCGCT17500.036.6551932
CTGTCGC16100.034.4328969
ACCGTCG1102.539245E-432.725488
GTCTTGC32050.030.2224241
CTCGCTC19650.030.075273
GGTATAC6650.029.3490871
CGCTCTG18550.029.1089155
GTATAGG9250.028.9142861
GTACCGA1606.971967E-527.1071431
GTATACG1907.328639E-626.631581
GTGTTAG23350.026.3138581
GTATTAT11100.026.0489081
GTATAAT9600.024.8482131
GTGTATA18450.024.6829281
GGGTAAT11900.024.297721
GTATTAG11650.024.1986521
GTGTAAC12400.023.3179741
Produced by FastQC (version 0.11.5)