FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431301
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17050.042.6515358
GTCTTGC31400.039.4589351
GTCTCGC16200.038.241071
TCGCTCT17550.036.10514
CTGTCGC22450.033.3530589
TCTCGCT19750.032.092152
CTCGCTC20600.030.4099033
CGCTCTG19450.029.6160435
TTGCTCT44900.025.9795514
GTATTAG12000.025.2124271
GTCTTAC14400.025.0123271
GTATTAA11050.024.772391
TTCACCG11100.024.6500235
GTCTCAC40300.024.1311
CTTGCTC44100.023.5118543
GTGTTAG23300.023.1873931
GTACTAA7500.023.0513611
GTACTAG7550.022.8987031
TCTGTCA47100.022.6244758
TCTTGCT50000.022.615852
Produced by FastQC (version 0.11.5)