FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3279213
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13800.043.8251388
GTCTTGC32400.035.3465841
GTCTCGC14300.034.2506031
CTGTCGC19000.032.209349
TCGCTCT16850.029.9117034
GGTATAC6050.029.7632241
CCGTGTT10900.029.063159
TTGCTCT43950.028.1781544
TATACCG1555.769571E-527.8712165
ACCGTGT10400.027.691718
GTGTTAG22850.026.7934131
TAATACT10800.026.667314
GTATTAA9150.025.9769521
GTGTAAC10150.025.5465221
TTCACCG9750.025.1079165
GTCTCAC38500.024.6949711
TCTCGCT18250.024.4657152
CTTGCTC43300.024.444013
GTTCTAC9850.024.1308731
AACCGTA2101.6424212E-523.9994817
Produced by FastQC (version 0.11.5)