FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145429
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11850.040.707218
CTGTCGC13800.035.4768189
GTCTCGC14350.033.234091
GTCTTGC31050.030.0206321
TCGCTCT14350.029.0998654
CTCGCTC16800.026.592333
GTATCGC1701.05559986E-425.5031641
TCTCGCT17800.024.693962
GTCTTAC12750.024.369691
GTGTTAG24200.023.588671
GGGTTTC56150.022.7779831
GTATTAC8300.022.6353381
GTGTAAG15050.022.5659261
GTATCGT2252.7230304E-522.4805681
TTGCTCT43750.022.3808044
GTTTCAC51350.022.3115983
GTATTGG11150.022.0341261
CGCTCTG16700.021.9871315
TTACACT17700.021.9652084
GTCCGTA1650.002652882821.8966561
Produced by FastQC (version 0.11.5)