FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145429
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13250.035.863088
GTCTCGC12550.031.5668931
GTCTTGC29100.029.950581
CTGTCGC17600.028.6350549
ACCGTGT10500.028.1134438
GGTATAC6700.026.8768311
TCGCTCT15150.026.6139134
GTGTTAC13900.025.910041
TCTCGCT16700.024.1495172
TTCACCG11250.023.6800445
GTTTCAC49550.023.2492873
GTGTAAT13100.023.0935571
GTATTAT10700.022.8880081
GTCTCAC38050.022.7163961
GTATTAC9250.022.582351
CTCGCTC16300.022.5276493
TCACCGT13850.022.3538326
CCGTGTT11450.022.0077259
GGGTTTC56050.021.9752251
GTATAGA9850.021.9380441
Produced by FastQC (version 0.11.5)