FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150913
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16000.041.8657721
TCTGTCG14200.039.0439538
GTCTTGC31300.035.898511
TCGCTCT16300.035.781274
CTGTCGC18200.033.2301069
CTCGCTC18150.032.9275783
TCTCGCT19450.032.5839772
GTATTAA10450.029.6379621
CGCTCTG19450.029.2459555
ACTGTCG3255.638867E-1126.5857738
TTGCTCT44600.026.3154644
GTATAGA11350.026.0186161
GTCTCAC38400.025.5096111
GTGTTAG21650.024.9516641
GTCTTAC13000.024.932471
CTCGATC11250.024.321068
GTTTCAC49150.024.1723423
GGGTTTC53350.024.1665551
ACCATGT41600.023.712578
CTTGCTC43100.023.5559223
Produced by FastQC (version 0.11.5)