FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564173
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13350.040.987788
GTCTCGC15500.039.0173261
GTCTTGC31200.037.1519131
CTGTCGC17600.033.953739
TCTCGCT18250.031.5600012
TCGCTCT18150.031.338094
CTCGCTC20800.027.3454973
CGCTCTG20350.026.5349925
GTGTAAC10150.025.5356181
GTCTTAC12300.024.5840851
GTGTTAC16050.023.7744641
GTGTAAA21700.023.2245981
TTGCTCT43050.023.0795864
GTTTCAC52450.023.061413
GTATAAT10000.023.0388031
GTATAAG10100.022.8106941
GTGTAGA19050.022.2980521
GTCTCAC41100.022.2470171
GTATTAA11350.021.567161
GGAGTGC83050.021.4997845
Produced by FastQC (version 0.11.5)