FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4111853
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16250.042.088088
CTGTCGC20400.034.584769
GTCTTGC35350.034.188291
GTCTCGC18250.028.7229081
TCGCTCT19350.028.2806994
GTACTAT9550.025.9199561
GTCTTAC15600.025.2015881
CGCTCTG23000.024.7279515
GTGTAAT16600.024.5605851
GTGTTAC17200.024.5503851
TCTCGCT21550.024.3911972
GTGTAAA22700.023.7336541
GTATTAG13500.023.7289031
CTCGCTC23300.023.4863323
GTGTTAG27400.023.1167851
GTATAGG9500.022.9909211
TTACACT21400.022.8798224
GGAGTGC94150.022.6339445
GTACGGT2953.7268546E-722.2115691
GTGTAGT22650.022.178881
Produced by FastQC (version 0.11.5)