FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3687882
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14500.042.202648
GTCTCGC16350.036.9870951
TCGCTCT17150.035.6815344
CTGTCGC18450.035.118419
GTCTTGC34450.033.4363941
CGCTCTG19000.031.0705495
CTCGCTC19150.030.827183
TCTCGCT19750.030.6196982
GCGTAGA2955.2568794E-1026.844751
GTCTCAC42700.025.121591
TTGCTCT47250.023.9213964
GTATTAC7950.022.6392251
TCTGTCA46950.022.2341798
GTATTAG12350.022.1516111
GTGTAGC16150.021.8429991
GTGTTAG25600.021.6540661
CTTGCTC46750.021.5593223
GGAGTGC83950.021.4391735
GTGTAAA22200.021.4032461
GTATAAT11500.021.2848111
Produced by FastQC (version 0.11.5)