FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3238960
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14550.040.1025471
TCTGTCG13250.036.950048
GTCTTGC29350.032.6432531
TCGCTCT16350.032.145014
TCTCGCT17700.029.699232
GTGTTAG21250.028.814421
TACACCG2802.8558134E-1028.284155
CTGTCGC19250.027.3032289
CGTTTCG1856.144306E-627.2425779
CTCGCTC19150.026.3219593
GTACCGC2259.643118E-725.612821
CGCTCTG19150.025.5651655
TGTACCG2651.4744728E-724.4514775
GAGTGCA87050.024.1510476
GGAGTGC80650.023.835015
GTGTAAC9150.023.6183761
CTGGAGT92450.023.0554583
GTATTAT9150.022.8310971
GTCTTAC12000.022.8114171
GGCTGGA108650.022.7412471
Produced by FastQC (version 0.11.5)