FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581249
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15050.043.054928
GTCTCGC15200.042.655051
GTCTTGC32000.034.6690751
CTGTCGC19400.034.5142149
TCGCTCT18100.033.014924
GGGTACG2900.032.2936331
TCTCGCT20150.031.09182
CTCGCTC20700.029.915723
GTCTCAC40250.029.1738171
CGCTCTG20500.028.4473555
GTATTAC7500.026.89481
GTGTTAG25100.026.6919781
GTTTCAC51000.026.2611733
GTATTAA10750.026.1353111
GTCTTAC12750.025.9907721
GGGTTTC56350.025.952171
TCTGTCA45900.024.6265628
ACCGTGT11400.023.9991328
TCTTGCT48950.023.8321912
CTTGCTC46450.023.7179473
Produced by FastQC (version 0.11.5)