FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329270
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12100.040.4595958
TCGCTCT13950.034.061814
GTCTTGC31700.033.3843571
GTCTCGC14450.033.380431
CTGTCGC18250.028.7977129
TTCACCG8900.027.5034315
GCGTAGT3204.5474735E-1126.9970441
CTCGCTC16300.026.9426443
GGTATAC6550.026.378791
GTGTAGC15050.025.831061
TTGCTCT42300.025.5298864
CGCTCTG17300.025.3852675
GTTTCAC49650.025.3756283
GTCTTAC12500.025.3412251
GGGTTTC54700.024.874791
GTGTAAC11100.024.6459481
ACCGTGT11600.024.2049738
GTGTAAT13700.024.1725351
TCTCGCT18000.023.997372
GTCTCAC37150.023.8358821
Produced by FastQC (version 0.11.5)